EasyMount Multichannel Ussing Chambers

EasyMount Multichannel Ussing Chambers

The EasyMount chamber systems are ideal for studies requiring electrophysiological measurement of transmembrane resistance. While visually similar to the NaviCyte Vertical chambers, EasyMount chambers are different in that they use inserts to position and secure tissues or culture cups within the chamber body.

  • 2, 4, 6, and 8 chamber systems available
  • Tissue and cell culture inserts for all applications
  • Built-in temperature control block
  • Needle valve gas flow control manifold
  • Ag/AgCl voltage and current electrodes
  • Series connection for multiple systems

 

How to order EasyMount Ussing Systems (Please see Item Listing):
(Only available as full system)
1. Select System
    Each system includes heater block/stand; chambers; needle valves; electrodes; amplifiers; and software.
2. Select Inserts
    P2300 series inserts fit the Standard and Rat systems; P2400 series inserts fit the Low Volume systems.
3. Select FN-15 Filling Needles
    Electrode tips; and thermocirculator (if needed)
4. Select P2300 Chamber Caps
    If using radioligands or toxic chemicals

Please see Full Description for complete information about EasyMount Multichannel Ussing Chambers.

 

Item# Description U.S. List Price Quantity
69-3217 EM2-C: 2-channel EasyMount system for tissue & cultured cells, standard volume (2.0 - 8.0 ml)
69-3220 EM4-C: 4-channel EasyMount system for tissue & cultured cells, standard volume (2.0 - 8.0 ml)
69-3223 EM6-C:: 6-channel EasyMount system for tissue & cultured cells, standard volume (2.0 - 8.0 ml)
69-3226 EM8-C: 8-channel EasyMount system for tissue & cultured cells, standard volume (2.0 - 8.0 ml)
69-3253 EM2-C-HV 2-channel EasyMount system for tissue & cultured cells, high voltage, standard volume (2.0 - 8.0 ml)
69-3256 EM4-C-HV 4-channel EasyMount system for tissue & cultured cells, high voltage, standard volume (2.0 - 8.0 ml)
69-3259 EM6-C-HV 6-channel EasyMount system for tissue & cultured cells, high voltage, standard volume (2.0 - 8.0 ml)
69-3262 EM8-C-HV 8-channel EasyMount system for tissue & cultured cells, high voltage, standard volume (2.0 - 8.0 ml)
69-3219 EM2-R: 2-channel EasyMount system for rat intestinal tissue (2.0 - 8.0 ml)
69-3222 EM4-R: 4-channel EasyMount system for rat intestinal tissue (2.0 - 8.0 ml)
69-3225 EM6-R: 6-channel EasyMount system for rat intestinal tissue (2.0 - 8.0 ml)
69-3228 EM8-R: 8-channel EasyMount system for rat intestinal tissue (2.0 - 8.0 ml)
69-3255 EM2-R:-HV 2-channel EasyMount system for rat intestinal tissue, high voltage (2.0 - 8.0 ml)
69-3258 EM4-R:-HV 4-channel EasyMount system for rat intestinal tissue, high voltage (2.0 - 8.0 ml)
69-3261 EM6-R:-HV 6-channel EasyMount system for rat intestinal tissue, high voltage (2.0 - 8.0 ml)
69-3264 EM8-R:-HV 8-channel EasyMount system for rat intestinal tissue, high voltage (2.0 - 8.0 ml)
69-3218 EM2-LV: 2-channel EasyMount system for tissue & cultured cells, low volume (0.5 - 5.0 ml)
69-3221 EM4-LV: 4-channel EasyMount system for tissue & cultured cells, low volume (0.5 - 5.0 ml)
69-3224 EM6-LV: 6-channel EasyMount system for tissue & cultured cells, low volume (0.5 - 5.0 ml)
69-3227 EM8-LV: 8-channel EasyMount system for tissue & cultured cells, low volume (0.5 - 5.0 ml)
69-3254 EM2-LV-HV: 2-channel EasyMount system for tissue & cultured cells, low volume, high voltage (0.5 - 5.0 ml)
69-3257 EM4-LV-HV: 4-channel EasyMount system for tissue & cultured cells, low volume, high voltage (0.5 - 5.0 ml)
69-3260 EM6-LV-HV: 6-channel EasyMount system for tissue & cultured cells, low volume, high voltage (0.5 - 5.0 ml)
69-3263 EM8-LV-HV: 8-channel EasyMount system for tissue & cultured cells, low volume, high voltage (0.5 - 5.0 ml)
69-3229 EM2-C-CE: 2-channel EasyMount system for tissue & cultured cells, CE (2.0 - 8.0 ml)
69-3232 EM4-C-CE: 4-channel EasyMount system for tissue & cultured cells, CE (2.0 - 8.0 ml)
69-3235 EM6-C-CE: 6-channel EasyMount system for tissue & cultured cells, CE (2.0 - 8.0 ml)
69-3238 EM8-C-CE: 8-channel EasyMount system for tissue & cultured cells, CE (2.0 - 8.0 ml)
69-3241 EM2-C-HV-CE: 2-channel EasyMount system for tissue & cultured cells, high voltage, CE (2.0 - 8.0 ml)
69-3244 EM4-C-HV-CE: 4-channel EasyMount system for tissue & cultured cells, high voltage, CE (2.0 - 8.0 ml)
69-3247 EM6-C-HV-CE: 6-channel EasyMount system for tissue & cultured cells, high voltage, CE (2.0 - 8.0 ml)
69-3250 EM8-C-HV-CE: 8-channel EasyMount system for tissue & cultured cells, high voltage, CE (2.0 - 8.0 ml)
69-3231 EM2-R-CE: 2-channel EasyMount system for rat intestinal tissue, CE (2.0 - 8.0 ml)
69-3234 EM4-R-CE: 4-channel EasyMount system for rat intestinal tissue, CE (2.0 - 8.0 ml)
69-3237 EM6-R-CE: 6-channel EasyMount system for rat intestinal tissue, CE (2.0 - 8.0 ml)
69-3240 EM8-R-CE: 8-channel EasyMount system for rat intestinal tissue, CE (2.0 - 8.0 ml)
69-3243 EM2-R-HV-CE: 2-channel EasyMount system for rat intestinal tissue, high voltage, CE (2.0 - 8.0 ml)
69-3249 EM6-R-HV-CE: 6-channel EasyMount system for rat intestinal tissue, high voltage, CE (2.0 - 8.0 ml)
69-3252 EM8-R-HV-CE: 8-channel EasyMount system for rat intestinal tissue, high voltage, CE (2.0 - 8.0 ml)
69-3230 EM2-LV-CE: 2-channel EasyMount system for tissue & cultured cells, low volume, CE (0.5 - 5.0 ml)
69-3233 EM4-LV-CE: 4-channel EasyMount system for tissue & cultured cells, low volume, CE (0.5 - 5.0 ml)
69-3236 EM6-LV-CE: 6-channel EasyMount system for tissue & cultured cells, low volume, CE (0.5 - 5.0 ml)
69-3239 EM8-LV-CE: 8-channel EasyMount system for tissue & cultured cells, low volume, CE (0.5 - 5.0 ml)
69-3242 EM2-LV-HV-CE: 2-channel EasyMount system for tissue & cultured cells, low volume, high voltage, CE (0.5 - 5.0 ml)
69-3245 EM4-LV-HV-CE: 4-channel EasyMount system for tissue & cultured cells, low volume, high volt
69-3248 EM6-LV-HV-CE: 6-channel EasyMount system for tissue & cultured cells, low volume, high voltage, CE (0.5 - 5.0 ml)
69-0956 Insert for EasyMount Diffusion Chamber - for Snapwell Cell Culture
69-1081 (P2302M) EasyMount insert, fits P2300 chamber, for Millicell cell culture cups, 8.75 mm dia., 0.60 cm2
69-1082 (P2302N) EasyMount insert, fits P2300 chamber, for Nunc/Anopore cell culture cups, ,8.0 mm dia., 0.50 cm2
69-1083 (P2302T) EasyMount insert, fits P2300 chamber, for Costar Transwell cell culture cups, 6.5 mm dia, 0.33 cm2
69-1084 (P2303) EasyMount insert, fits P2300 chamber, for juvenile mouse intestine, 2.8x4.5 mm, 0.10 cm2
69-1085 (P2303A) EasyMount insert, fits P2300 chamber, with pins, for juvenile mouse intestine or bladder, 2.8x4.5 mm, 0.10 cm2
69-0957 Insert for EasyMount Diffusion Chamber - for Mouse Intestine
69-0958 Insert for EasyMount Diffusion Chamber - for Rat and Larger Animal Intestines
69-0959 Insert for EasyMount Diffusion Chamber - for Mouse Trachea, Similar Tissues
69-0960 Insert for EasyMount Diffusion Chamber - for Small Tissues (I.e., Biopsies)
69-1086 (P2308) EasyMount insert, fits P2300 chamber, for very small tissues (lower mouse trachea), 1 mm dia, 0.008 cm2
69-0961 Insert for EasyMount Diffusion Chamber - with Mounting Pins
69-0962 Insert for EasyMount Diffusion Chamber - with Mounting Pins
69-1087 (P2311A) EasyMount insert, fits P2300 chamber, with mounting pins, 5.7 mm dia, 0.26 cm2
69-0963 Insert for EasyMount Diffusion Chamber - with Mounting Pins
69-0964 Insert for EasyMount Diffusion Chamber - with Mounting Pins
69-0965 Insert for EasyMount Diffusion Chamber - with Mounting Pins
69-0966 Insert for EasyMount Diffusion Chamber - with Mounting Pins
69-1088 (P2316) EasyMount insert, fits P2300 chamber, with o-ring, for skin, 5.0 mm dia, 0.20 cm2
69-1089 (P2317) EasyMount insert, fits P2300 chamber, with o-ring, for synthetic membranes, 10.8 mm dia, 0.93 cm2
69-1090 (P2318) EasyMount insert, fits P2300 chamber, with o-ring, for frog skin, 11.3 mm dia, 1.00 cm2
69-1091 (P2319) EasyMount insert, fits P2300 chamber, with spacer ring, for studying mucus layer,12.7 mm dia, 1.26 cm2
69-1092 (P2403) EasyMount insert, fits P2400 chamber, for small tissue specimens, 2.8x4.5 mm, 0.10 cm2
69-0970 Insert for EasyMount Diffusion Chamber - for Rat and Larger Animal Intestines
69-0971 Insert for EasyMount Diffusion Chamber - for Mouse Trachea, Similar Tissues
69-0969 Insert for EasyMount Diffusion Chamber - for Mouse Intestine
69-1093 (P2408) EasyMount insert, fits P2400 chamber, for small tissues (biopsies), 0.8 mm dia, 0.005 cm2
69-1094 (P2408A) EasyMount insert, fits P2400 chamber, for small tissues (biopsies), 0.5 mm dia, 0.002 cm2
69-1095 (P2408B) EasyMount insert, fits P2400 chamber, for small tissues (biopsies), 1.0 mm dia, 0.008 cm2
69-0973 Insert for EasyMount Diffusion Chamber - with Mounting Pins
69-0975 Insert for EasyMount Diffusion Chamber - with Mounting Pins
69-0976 Insert for EasyMount Diffusion Chamber - with Mounting Pins
69-1096 (P2300CAP) Chamber caps for P2300 chamber, set of 2
69-1097 (FNS152) Two syringes with filling needles
69-1098 (FNS15) Replacement filling needle
69-0990 Electrode Tips, pkg of 20
69-0991 Electrode Tips, pkg of 50
69-0992 Electrode Tips, pkg of 100
72-4924 (TC-120) Thermocirculator, 5 Liter Plastic Bath with Lid, 120 V, 60 Hz
72-4921 (TC-120) Thermocirculator, 5 Liter Plastic Bath with Lid, 220 V, 50 Hz
64-2244 (WB-KIT) TC-120 Thermocirculator Tubing Kit for EasyMount/Navicyte/Strathkelvin
69-0993 EasyMont Diffusion Chamber Model P2300
69-0995 EasyMont Diffusion Chamber Model P2400
69-0987 Voltage and Current Electrode Set
69-0988 Voltage Electrode Set
69-0989 Current Electrode Set
69-3147 (P2024-36) Electrode lead set, 36 in, 1 ea
69-0950 Single Channel Electrode Input Module and Dummy Membrane
69-0952 6-Channel Dummy Membrane for VCC MC6
99-0778 Replacement O-rings for EasyMount P2300 chambers, pack of 6
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The EasyMount chamber systems consist of 2 to 8 vertical Ussing chambers, a heater block/support stand, needle valves for adjustment of gas flow (for oxygenation and gas lift stirring), and Ag/AgCl voltage/current electrodes for measuring transepithelial voltage and for passing current. Tissue specific inserts are ordered separately.

Multichannel setups

All EasyMount chambers (P2300 and P2400,) use the same stands and electrodes. Stands are available to support 2, 4, 6, or 8 chambers and can be readily ganged to allow for higher count applications. Stands have an incorporated heater block that warms all chambers to the same temperature. A needle valve assembly (one set per chamber) is mounted on the rear of the heater block for regulation of the gas flow.

EasyMount chambers

EasyMount chambers are a two piece assembly using an insert to secure and position tissues or culture cups. Tissues are placed in the chamber by loosening a thumbscrew, sliding the tissue holding insert into the space between the chamber halves, and retightening the thumbscrew. Chambers do not need to be removed from the heater block, nor are the electrodes disturbed, when placing an insert. This easy and rapid replacement process makes the EasyMount system an excellent choice for high-throughput applications.

Chambers are available in two styles; the P2300 is the standard chamber and accommodates a large variety of inserts and the P2400 is designed for low-volume applications. The P2300 has chamber caps available for sealing the chamber when radioligands or toxic chemicals are used.

EasyMount Inserts

EasyMount inserts are available to mount a variety of tissues and can also accommodate cell culture cups. Each chamber type has its own supporting family of inserts.

The P2300 Series inserts are available to accommodate Snapwell, Millicell, Nunc, and Transwell culture cups. Inserts are also available with pins or o-rings in both oblong and round formats.

P2400 Series inserts do not support culture cups but are available with pins or o-rings in both oblong and round formats.

Electrodes and Accessories

Electrodes are available in sets comprised of two sintered Ag/AgCl pellet electrodes for voltage sensing (black), two Ag wire electrodes for current passing (white), and eight electrodes tips. The number of sets provided is dependent on the system purchased, however, one electrode set is required for each chamber.

Replacement current and voltage electrodes are available, as are electrode tips. Additional accessories include electrode lead sets and chamber filling needles.

Specifications
DOCUMENT DESCRIPTION
Warner's Guide to Ussing Chamber Systems (091022) WLR.pdfWarner's Guide to Ussing Chamber Systems
Corning-Snapwell-Transwell Instruction Manual1.pdfCorning Guide to Snapwell