Confocal Imaging Chambers (RC-30, RC-30HV, and RC-30WA)

Confocal Imaging Chambers (RC-30, RC-30HV, and RC-30WA)

The RC-30 is a low profile closed bath chamber specifically designed for confocal imaging applications. Compatible with either upright or inverted microscopes.

  • Option of confocal imaging with continuous perfusion
  • Accommodates both upright and inverted microscopes
  • 17.7 mm diameter viewing aperture (RC-30, RC-30HV)
  • 25.0 mm diameter viewing aperture (RC-30WA)
  • User defined cross-sectional viewing area
  • User defined bath volume with fast fluid exchange
  • Three available gasket thicknesses
  • Resistive heating option on chamber base

Item# Description U.S. List Price Quantity
64-0707 CS-22/40 Coverslips, use with RC-22, RC22C, RC-24, RC-24E, RC-24N, RC-26, RC-26G, RC-26GLP, RC-30, and RC-50, pkg of 50
64-0706 CS-22/30 Coverslips, 22 x 30 mm for use with RC-30 Chambers, pkg of 50
64-1500 (CS-40R) Round cover glass, #1 thickness, 40 mm, 50 pack
64-1499 (CS-30R15) Round cover glass, #1.5 thickness, 30 mm, 90 pack
64-0754 (PE-90/10) Polyethylene tubing, PE-90, 10 ft
64-0335 Replacement Gaskets for Blank, 375 um Thickness, pkg of 10
64-0334 Replacement Gaskets for Blank, 250 um Thickness, pkg of 10
64-0341 Replacement Gaskets for Blanks, 150 um Thickness, pkg of 10
64-0333 Replacement Gaskets for Slotted Bath, 375 um Thickness, pkg of 10
64-0332 Replacement Gaskets for Slotted Bath, 250 um Thickness, pkg of 10
64-0343 Replacement Gaskets for Slotted Bath, 150 um Thickness, pkg of 10
64-0331 Replacement Gaskets for Large Bath, 375 um Thickness, pkg of 10
64-0330 Replacement Gaskets for Large Bath, 250 um Thickness, pkg of 10
64-0342 Replacement Gaskets for Large Bath, 150 um Thickness, pkg of 10
64-0274 Heater Elements (2) for PH (Heater) Platforms with Mounting Pads and Screws, RH-2
64-0103 SF-28 Slow Flow In-Line Solution Heater
64-0102 SH-27B Solution In-Line Heater
64-0107 TA-29 Replacement Cable with Bead Thermistor
64-0106 Model CC-28 Cable Assemblies with Connector for TC-324B/344B
64-2401 TC-344C Heater Controller, Dual Channel
64-2400 TC-324C Heater Controller, Single Channel
64-0380 Series 30 stage adapter for Use with Nikon E400 / E600 / E800, Olympus BX-40 / BX-50
64-0377 Series 30 stage adapter for Leica Galvo Z Stage
64-0329 For Prior and Ludl Motorized Stage
64-0328 Stage Adapters for Leica MIRB/E with 3-Plate Mechanical Stage
64-0326 Stage Adapters for Zeiss Axiovert 211 x 230 Specimen Stage and for Leica DMIRB/E with Plane Stage
64-0325 Stage Adapters for Olympus IMT-2/IX50/IX70/BX50WI
64-0324 RC-30 stage adapter for Nikon
64-0322 Stage Adapters for Nikon Diaphot/TE200/TE300
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The RC-30 and RC-30HV are closed bath low profile chambers incorporating special features for microscopic confocal imaging.

These features include user defined bath geometry and a large viewing area. A 22x30 mm, #1 glass coverslip forms the top of the chamber, while a 22x40 mm, #1 coverslip forms the bottom, respectively, creating a closed bath. The closed design allows the chamber to be inverted in the mounting platform enabling its use with both inverted or upright microscopes. The final viewing aperture is 17.7 mm diameter.

The RC-30WA is a wide aperture version of the RC-30HV. It uses a 40 mm round, #1 coverslip for its bottom surface and a 30 mm round, #1 coverslip for the top. The final viewing aperture is 25 mm diameter.

Variable Bath Volume/Fast Perfusate Exchange: The side walls of the RC-30 bath is formed by a silicone gasket sandwiched between the top and bottom coverslips. Wall height (i.e. spacing between the two coverslips) can range from 150-1000 µm and is defined by the thickness and number of gaskets used. Blank gaskets are supplied with the chamber permitting the development of the customized bath geometry required for individual applications.

Pre-cut gaskets are also supplied with the chamber; one cut wide to allow full use of the aperture window (17.7 mm diameter) and a narrow, slotted version for fast solution exchange. The bath volumes for these pre-cut, 250 µm thick gaskets are 26 µl for the slotted cut and 133 µl for the wide cut.

Large Viewing Area
The viewing aperture in the chamber housing is 17.7 mm in diameter. The chamber bottom is beveled to permit maximum access to the bottom coverslip.

The chamber can be mounted in the stage adapter to accommodate both upright and inverted microscopes.

Heater Version
The RC-30HV and RC-30WA include a pair of resistive heater elements mounted to the chamber base. These resisters are compatible with Warner TC-324C and TC-344C temperature controllers which be ordered separately along with the CC-28 cable.

All versions of the confocal imaging chambers require special Series 30 microscope stage adapters which must be ordered separately.

Confocal imaging chambers are supplied with six packages of pre-cut gaskets: two large area bath gaskets (GS-30L/10, GS-30L/15), two slotted bath gaskets (GS-30S/10), and two blank gaskets (GS-30B/10, GS-30B/15) for user customized chamber areas. Also included is a special tool used for mounting the top plate, polyethylene tubing (PE-90/10, 10 feet.), and 22x30 mm and 22x40 mm #1 glass coverslips (CS-22/30 and CS-22/40, pkg. of 50). The RC-30WA includes one box each of 30 mm round coverslips, (CS-30R, and 40 mm round coverslips, CS-40R).

Warner Instruments offers 150, 250,375 µm thick gaskets which are listed in the order information section.

Thickness150 um150 um150 um
Physical Dimensions (H X D) 9.5 x 82 mm
Weight <90 g, assembled with optional heaters
Base and Pressure Plate Aluminum, black anodized
Top Coverslip Plate Polycarbonate
Gaskets Silicone rubber
Input/Output Tubing PE-90
Coverslips (#1) Top: 22 x 30 mm; Bottom: 22 x 40 mm
Microscope Stage Adapters* All major models:
Leica, Nikon, Zeiss, Olympus, Prior, Ludl, Burleigh
*Note: Uses Series 30 stage adapters. For exterior dimensions use exterior dimension information for corresponding Series 20 adapters.

Integrated Components Brochure_06_7_11.pdfIntegrated Components for Live Cell Imaging Brochure
perfusion_strategies.pdfPerfusion Strategies (Leinders-Zufall)