Computer Controlled Epithelial Voltage/Current Clamp Systems

Computer Controlled Epithelial Voltage/Current Clamp Systems

This Multi-Clamp system is a combined voltage/current clamp and data acquisition system in one compact microcomputer controlled unit. In its full configuration it can be used to control and collect electrophysiological data from up to 6 Harvard/NaviCyte horizontal or vertical diffusion chambers. Diffusion chamber systems sold separately.

Item# Description U.S. List Price Quantity
66-0025 24 Leads Voltage/Current Electrode Manifold, 2mm pin sockets
69-0302 6-Channel Voltage/Current Clamp System with Software
69-0301 4-Channel Voltage/Current Clamp System with Software
69-0300 2-Channel Voltage/Current Clamp System with Software
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This Multi-Clamp system is a combined voltage/current clamp and data acquisition system in one compact microcomputer controlled unit. In its full configuration it can be used to control and collect electrophysiological data from up to 6 Harvard/NaviCyte horizontal or vertical diffusion chambers. Diffusion chamber systems sold separately.


A second system configuration that consists of a precision gas delivery system with 12 precision gas flowmeters that can be used with either the 6 channel Harvard/NaviCyte chamber vertical or horizontal diffusion chamber systems. The precision gas flowmeters and Harvard/NaviCyte diffusion chamber system is attached to a 600 x 400 mm stabilizing base. Also attached to this base is an electrode manifold for connecting each Ag/AgCl electrodes to their respective half-cells. Diffusion chambers systems sold separately.


For each individual diffusion chamber there is a separate analog to digital converter and an isolated current source. The fluid resistance as well as the electrodes potential is calculated at the beginning of the experiment and is maintained during the experiment. No manual adjustments need to be made during the experiment. While the experiment is in process, individual markers can be set to denote information such as the introduction of a substance.


The system provides independent chamber control in three different modes: Voltage Clamp, Current Clamp and Open Circuit. An individual change between these modes is possible during experiment at each time point. The current and voltage electrodes from each half cell are connected to the Multi-Clamp clamp via a electrode manifold which is then connected to the microcomputer system via a standard DB 25 pin cable. The data exchange between the PC and the Multi-Clamp is based on a serial connection (COM Port). No additional card has to be plugged into your PC! Depending on the number of available COM ports, one PC can handle several clamps. The Multi-Clamp systems are available in 2, 4 or 6-channel configurations and are fully compatible with the Harvard/


NaviCyte 6-channel horizontal and vertical diffusion chambers.


The Multi-Clamp System comes complete with an easy to use Windows® 95/98/NT based control and data acquisition software that allows for the individually control and monitoring of each chamber. Some of the software features of the Multi-Clamp Software include:


• Automatic compensation of fluid resistance Rf and electrode potential dPe during experiment


• Voltage clamp, current clamp or open circuit control for individual chambers with individual changing between modes


• Overlay of uni/bipolar current pulses with variable parameters for dynamic definition of Gt/Rt


• I/V and V/I curves with free adjust-able parameters


• Min. pulse duration 200 ms, max. amplitude ±250 µA for dynamic definition of Gt/Rt


• Alphanumeric display of measured parameters in form of table


• Ability to input marker and comments on display and save with data file


• Storage of data to hard disk and playback of existing data files


• Printout of parameters in variable timetable


• Data format compatible to MS Excel® or equivalent


• Continuous graphics display for different measurement parameters (selectable) Gt/Rt, dP0, I


• Experimental time up to 8 hours


• Auto back-up on hard disk every 10 minutes


All tissue data–resistance Rt/conductivity Gt, voltage dP0 (mV), and current I (µA/cm2 or µEq/hcm2)–are monitored in a numerical or graphical form that is separated for each chamber. A list showing the parameters (selectable) in parallel columns is also available.


Data is acquired and stored real time into the PC’s memory. This feature provides for fast updates to the display for ‘real time’ viewing. The data stored in the PC memory is automatically stored to the hard disk drive every 10 min. With this technique an experiment may be controlled and logged for as long as 8 hours. At the end of the experiment, all data is stored as a permanently file on the hard disk drive. The data format for data storage is compatible to MS Excel which allows a further analysis and custom graphing of the data.
Specifications