Coleman Superfusion Bath System

Coleman Superfusion Bath System

Suitable for a variety of applications involving the study of contraction and relaxation of smooth muscle preparations, the effect of drugs that interfere with these actions, as well as those that interfere with autonomic neurotransmission.

For questions or for help configuring a system, please contact Technical Services at 800-547-6766 or via email.

Item# Description U.S. List Price Quantity
73-2221 BASIC UNIT FOR SUPERFUSION BATH ACCORDING TO COLEMAN (4-POSITION) TYPE 840
73-2222 SUPERFUSION BATH ACCORDING TO COLEMAN
73-2917 DUAL HEAT EXCHANGER TO SUPERFUSION BATH COLEMAN
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The Coleman superfusion system is suitable for a variety of applications involving the study of contraction and relaxation of smooth muscle preparations, the effect of drugs that interfere with these actions, as well as those that interfere with autonomic neurotransmission. 

Features & Benefits

  • Suitable for virtually any tissue sample
  • Compact, allows the study of up to four tissue preparations in parallel
  • Rapid removal of potentially toxic metabolites
  • Obviates the necessity of repeated washing procedures
  • Simple reclaimation of perfusate for recirculation or collection

Applications

  • Evaluation of the potency of labile substances
  • Determining the rates of onset and offset of drug action
  • Evaluation of spasmogenic and spasmolitic agents


Setup and Operation

 

The Coleman Superfusion Bath System consists of a main stand that can receive up to eight single superfusion chambers. The horizontally split superfusion chambers are mounted on a horizontal support rod according to the number of channels required.  All additional parts (tissue holder, dual heat exchanger, road for mounting the transducer) are fixed on the chamber.   A stand for 4-position superfusion system is available????

The tissue is located in one of these chambers. Each chamber is equipped with electrical stimulation capability, a rod for fixing the force transducer and an optional two-channel heat exchanger.  The lower part of the chamber can be swung away to simplify access to the preparation. The lower end of the tissue is held by a stainless steel hook. The thread attached to the top of the tissue passes through an opening at the top of the chamber for direct connection to the force transducer. The superfusion solution is fed by a multi-channel roller pump (peristaltic pump) through a stainless steel cannula to the thread; it runs down the thread and superfuses the tissue.

Two separate solution paths are available for superfusion: with and without test substance. Two platinum wires for field stimulation are placed parallel to the tissue to form the stimulation field (stimulation electrodes). The solution running down provides electrical contact between the electrodes and the tissue.

The superfusate is thermostated immediately before entering the chamber by a two line heat exchanger supplied from a water thermostat. The heat exchanger is made of a tubing coil that can be easily replaced. A collecting trough is located underneath the superfusion chambers for collecting of the used solution; alternatively the superfusate can be collected from the chamber outlet for recirculation.

Note: The superfusion chambers are made from a Plexiglas acrylate block  and do not require thermostating owing to the low thermal conductivity of the material used. 

 

The upper part of the chamber receives the tissueholder. The lower part can be swung away to simplify access to the preparation. The lower part is funnelshapedto allow the collection of theefluate for reperfusing, perfusing thenext tissue or bioassay analysis.

The isometric force transducer is fixed onthe rod using a Vernier control.The tissue holder is a separate partthat is installed in the main superfusion chamber.

The holder can easily be removed to simplify the preparation.The holder consists of the body, the hook for fixing the tissue, the stainless steel cannulas for superfusion and the stimulation electrodes.

The tissue is attached using a thread to the tissue hook. The thread at the other end ofthe tissue must be attached to the transducer. A slide in the holder body allows easy positioning of the thread in the central hole.

The superfusion cannulae can be rotated in the mainbody to optimize the superfusion after the holder is in place into the chamber and the thread fixed to the transducer.

The stimulation electrodes are made of platinum and must be buckled to be close to the tissue. The electrodes do not touch the tissue but must be parallel and close enough to the tissue so that the superfusing solution makes electrical contact.

The dual heat exchanger consists of Tygon tubing loops inside a jacket tube connected to a thermocirculator.

 


 

Modes of Operation

Flow through mode: Each tissue is superfused individually with fresh solution or solution containing the test compound. The outcoming solution is collected and goes to waste or is stored for later bioassay analysis.

Recirculating mode: Each tissue is superfused individually. The outcoming solution after superfusion is collected and used for superfusing again in the same tissue.

Cascade mode: The first tissue is superfused with fresh solution and all the subsequent tissues are superfused with the outcoming solution of the preceding chamber.


Included items: Stand (capacity 8 chambers), frame, Plexiglas chambers (4), tisue holder, dual heat exchanger, rod for mounting transducer

 

Additional equipment required: Peristaltic pump, force transducer withVernier control, bridge amplifier for the force transducer, Data acquisitionACAD, electrical stimulator, thermocirculator if heat exchangers are used.

Specifications