Classic Ussing Systems (U9500, U9520)
The investigation of ionic transport across epithelial cells was greatly facilitated by the development of a chamber system designed by Dr. H. H. Ussing more than 40 years ago. Today this equipment bears his name. The U9500 ‘Classic’ design presented here varies little from Dr. Ussing’s original equipment which remains in wide use for the study of electrophysiological properties of epithelial tissues. A newer design, the U9520 ‘Classic with Inserts’ allows for insertion of cell culture or tissue supports and greatly increases the functionality of the classic Ussing chamber. The low initial cost of the Classic design, coupled with the modest cost of add-on components, makes this system an ideal option for classroom instruction and in-the-field research.
- Classic system as designed by Ussing
- Supports membranes from 2.5 to 15.5 mm wide
- Supports Snapwell, Transwell, and Multicell, culture cups
- Insert version available
How to order (Please see Item Listing):
1. Select Base Assembly with desired reservoir size
Systems include support stand with rods circulation reservoir; 3-prong clamp; U9303 Air/Gas Fitting Kit; U9975A Electrode Set and U9965EP Electrode Bridge Fitting Kit
2. Select chamber
Choices are 'Classic' or 'Classic with Insert'. Both available with or without drains
3. Select insert
If using 'Classic with Insert'
Please see the Full Description for complete information about Classic Ussing Systems.
Note: Chambers with drains are available, pleae call out technical support for more information.
The U9500 and U9520 are complete chamber systems consisting of an Ussing chamber, chamber support stand, circulation reservoir, 3-prong clamp, air/gas fitting kit, and electrode set. Both systems are virtually identical with the difference embodied in the actual chamber.
U9500 'Classic' Ussing Chambers
The U9500 'Classic' chambers are machined from solid acrylic into two halves and have vertical and horizontal ports in each half for connection to the circulation system and for making electrical connections.
The face of one chamber-half is imbedded with sharp stainless-steel pins which mate with corresponding holes in the other chamber-half face. These pins allow for puncturing and positioning of an epithelium membrane within the chamber. Chambers are available in a variety of sizes and pin configurations. In the 'Classic' design, a separate chamber must be purchased for each different desired pin configuration. This is a good choice if you intend to study a single tissue type as the assembly is direct and simple.
U9520 'Classic with Inserts' Ussing Chambers
The U9520 'Classic with Inserts' chamber is functionally similar to the U9500 except that it makes use of low-cost adapters (inserts) to position and support tissues or cultured cells within the chamber body. Inserts are interchangeable and permit the researcher to investigate a variety of tissues without having to purchase a separate chamber for each different tissue. The U9520 'Classic with Inserts' chamber is mounted in the standard support stand and procedures for experimentation are the same as with the 'Classic' system.
Chamber and Insert Pin Configurations
U9500 chambers are available with pinned openings in diameters from 3.8 to 13.5 mm and in slot widths from 1.6 to 3.5 mm to accommodate a variety of tissue dimensions. The choice of pin configuration depends on the epithelia under study. The specified diameter designates the main opening to which the buffer makes contact with the membrane.
U9520 chamber inserts are available with openings in diameters from 3.8 to 13.5 mm. Inserts are also available for culture cups from Falcon, Millicell, Snapwell, and Transwell.
Both U9500 and U9520 chambers are available with and without drains.
Circulation Reservoirs, 3 Volumes
Available in 4, 10, and 20 ml capacities, circulation reservoirs are two sided, hand-blown apparatus made from borosilicate glass. Each side has two outlets at the base for connection to the perfusion fittings on the associated Ussing chamber-half. Each side also has a separate air/gas inlet to drive the circulation system. Gas, commonly a 95% O2/5% CO2 mixture, is forced under low pressure into these inlets and allowed to come into contact with the buffer solution. The rising bubbles drive circulation and oxygenate the buffer. This method is commonly referred to as a 'gas-lift' perfusion system.
Each horizontal reservoir arm has either an inlet or outlet port for access to the water jacket. Water at the desired temperature is pumped through the jacket and allowing thermoregulation of the perfusion solutions. Glass condensers are provided to minimize evaporative solution losses from the top.
Electrode Sets and Agar Bridge Fittings
Each System is supplied with a U9975A Electrode Set comprised of 4 electrodes, 2 Ag-AgCl pellet electrodes (for voltage) and 2 Ag wire electrodes (for current). Electrodes include 1 meter (3 feet) of wire and a connector to plug into the amplifier headstage.
Also supplied are Luer taper fittings that screw onto the electrodes to provide an agar salt bridge connection into the chamber. Fittings can be filled with agar either before or after assembly onto the electrodes.
|Warner's Guide to Ussing Chamber Systems (091022) WLR.pdf||Warner's Guide to Ussing Chamber Systems|
|U-9500 (1999.07.09).pdf||U-9500 User's Manual|
|U-9500CU (1999.07.09).pdf||U-9500CU User's Manual|
|U-9504 - 9513 (2003.06.19).pdf||U9504-9513 Classic Ussing Overview|
|Corning-Snapwell-Transwell Instruction Manual1.pdf||Corning Guide to Snapwell|