Classic Bilayer Cups and Chambers

Classic Bilayer Cups and Chambers

This 2-part system consists of a black Delrin chamber and a cuvette of either polystyrene, polysulfone or Delrin (cups purchased separately). Cups and chambers are designed such that addition of equal volumes to the cup and chamber results in a balanced solution height, minimizing any pressure gradients across the bilayer membrane.

  • Cuvettes in Delrin, Polystyrene, or Polysulfone (colored white, translucent or honey, respectively)
  • Precision Machined Apertures 150, 200 or 250 µm
  • Viewing Window
  • cis and trans stirbar wells
  • Stirbars Included with each cup and chamber
  • Shipments from Stock

Item# Description U.S. List Price Quantity
64-0451 (BCH-M13) Bilayer chamber, 1.0 ml working volume, with stirbar, without cuvette
64-0404 CP13A-150 13 mm Polystyrene Cuvette with 150 µm Aperture
64-0403 CP13A-200 13 mm Polystyrene Cuvette with 200 µm Aperture
64-0402 CP13A-250 13 mm Polystyrene Cuvette with 250 µm Aperture
64-0410 CD13A-150 13 mm Delrin Cuvette with 150 µm Aperture
64-0409 CD13A-200 13 mm Delrin Cuvette with 200 µm Aperture
64-0408 CD13A-250 13 mm Delrin Cuvette with 250 µm Aperture
64-0416 CF13A-150 13 mm Polysulfone Cuvette with 150 µm Aperture
64-0415 CF13A-200 13 mm Polysulfone Cuvette with 200 µm Aperture
64-0414 CF13A-250 13 mm Polysulfone Cuvette with 250 µm Aperture
64-0453 (BCH-M22) Bilayer chamber, 3.0 ml working volume, with stirbar, without cuvette
64-0407 CP22A-150 22 mm Polystyrene Cuvette with 150 µm Aperture
64-0406 CP22A-200 22 mm Polystyrene Cuvette with 200 µm Aperture
64-0405 CP22A-250 22 mm Polystyrene Cuvette with 250 µm Aperture
64-0413 CD22A-150 22 mm Delrin Cuvette with 150 µm Aperture
64-0412 CD22A-200 22 mm Delrin Cuvette with 200 µm Aperture
64-0411 CD22A-250 22 mm Delrin Cuvette with 250 µm Aperture
64-0419 CF22A-150 22 mm Polysulfone Cuvette with 150 µm Aperture
64-0418 CF22A-200 22 mm Polysulfone Cuvette with 200 µm Aperture
64-0417 CF22A-250 22 mm Polysulfone Cuvette with 250 µm Aperture
64-0420 MAG-13 Stir Bars 2x5mm for 13mm Cuvettes. pkg. of 5
64-0421 MAG-22 Stir Bars 2x7mm for 22mm Cuvettes, pkg. of 5
64-1729 (BCH-13TH) Replacement thumb screw and rubber plug for BCH-13
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The Basics

Cups and chambers from Warner Instruments are designed such that addition of equal volumes to the cis and trans sides results in a balanced solution height, thus minimizing mechanical gradients across the bilayer membrane.

Bilayer Cuvettes

Polystyrene has been a favored material for cuvettes for several years. The physical properties of this material make for a high quality membrane support. However, its poor resistance to organic solvents can lead to degradation of the aperture. Cuvettes have also been made from Delrin (acetyl resin) and many users report good membrane formation as well as easier maintenance and longer life. It should be noted, however, that Delrin cuvettes do not work equally well in all applications and with all users.

Warner Instruments now offers bilayer cups made from a new material, polysulfone. This material has many of the mechanical properties of polystyrene coupled with enhanced solvent resistance. The choice of material will depend on your application.

All cuvettes have a 0.5 mm well milled into their base to confine the motion of stirbars and to reduce mechanical noise artifacts. A 2 x 5 mm or 2 x 7 mm PTFE-coated stirbar is supplied with each cuvette.

Standard available aperture diameters are 150, 200 or 250 µm. Contact our offices for custom aperture sizes from 50 µm to 1 mm. The wall thickness at the aperture is 250 µm.

Bilayer Chambers

Chambers are made from black Delrin and also have a 0.5 mm recessed well milled into the floor of the non-cup side to confine the motion of stirbars and to reduce mechanical noise artifacts. A 2 x 5 mm or 2 x 7 mm PTFE-coated stirbar is supplied with each chamber.

Polyethylene centrifuge tubes (supplied) serve as intermediate wells for electrical connections between the headstage electrodes and the cup or chamber. Chambers include a nylon screw and rubber plug to secure the cups during use. The classic model has a polycarbonate window for viewing the aperture during membrane formation.

Classic Models BCH-13A and BCH-22A

Two classic models are offered: The BCH-13A is a small volume chamber (1.2 ml) with a 13 mm (1/2) diameter cuvette. The BCH-22A is a larger volume chamber with (5.0 ml) chamber with a 22 mm (7/8) diameter cuvette.

Specifications
DOCUMENT DESCRIPTION
Warner Bilayer Workstation brochure (rev 060601).pdfWarner Bilayer Workstation Brochure
Care and Use of Bilayer Cuvettes (2003.04.23).pdfCare and Use of Bilayer Cuvettes